Molecular Cloning and Sequence Analysis of Class II Chitinase Gene in Leymus chinensis

[Objective] The aim was to clone Class Ⅱ chitinase gene in Leymus chinensis which are growing in saline land in Heilongjiang Province,and lay a foundation for further researching the biological function and application of chitinase gene.[Method] Constructing cDNA library of Leymus chinensis leaves,its DNA sequence was determined and analyzed,the homology of chitinase gene and amino acid sequence was compared with that in the GeneBank.[Result] The total length cDNA segment was cloned,the length was 996 bp,the ORF was 768 bp among which,encoding 255 amino acids.It had the structural character of ClassⅡ chitinase gene because the encoded products lacked CBD and C-terminal extension region.The amino acid sequence had high homology compared with ClassⅡ chitinase gene of rye and wheat.The constructed recombinant vector pQE-LcChi2 can induce and express a protein of 27 KD,the result was consistent with deduced encoded product of pQE-LcChi2 gene.[Conclusion] LcChi2 gene has expressed function,which can express in E.coli.