Bacterioplankton diversity of Beilun Harbor in winter Ningbo

The bacterioplankton diversity in waters of Beilun harbor in November,2007,was studied using techniques of molecular biology.The clone library of 16S rDNA and the phylogenetic tree were constructed with extraction of bacterial DNA,PCR amplification of bacterial 16S rDNA by universal primers,molecular clone,sequencing of 16S rDNA fragments and sequence analysis.The results indicated that the bacterial diversity was high in that 34 belonged to 2 different published phyla and 6 belonged to unknown phylum.The dominant bacterial community was Proteobacteria,which accounted for 80% of the total.The bacterial community succession were listed as follows,β-Proteobacteria(32.5%),γ-Proteobacteria(25%),α-Proteobacteria(15%),e-proteobacteria(7.5%) and Firmicutes(5%).The result was much consistent compared with the microorganism diversity data published home and abroad in recent years.The phylogenetic results demonstrated higher bacterial diversity after 17 OTU phylotypes from 40 sequenced clones were selected and analyzed with Clustalw of DNAStar Software.