In vivo studies on the role of pU-VEGF-siRNA in the growth of tongue cancer xenograft

Objective:To assess influence of vector-based small interfering RNA(siRNA)targeting vascular endothe-lial growth factor(VEGF)for human tongue squamous cell carcinoma(Tca8113)xenografts in vivo.Method:Two vector-based siRNA targeting VEGF(PU-VEGF-siRNA1,PU-VEGF-siRNA2),and eukaryotic expression vector(experiment control)were transfected into Tca8113 cells by lipofectamine2000,and screened with G418.Non-transfected cell was used as negative control.Twenty nude-mice were divided into 4 groups randomly,and then the transfected cells and non-transfected cell were subcutaneous injected on the back of mice,respectively.The tumor volume,weight,and growth curve of every group were compared.The protein expression of VEGF was detected by immunohistochemical technique.Result:Compared with experiment and negative controls,the growth of xeograft was significantly reduced,VEGF expression decreased in two experiment groups(P 0.05).But there were no statistically difference of those between two controls(P 0.05).Conclu-sion:Vector-based siRNA targeting VEGF are efficient in suppressing the growth of Tca8113 cell xenografts in vivo.