Effect of Mitochondrial Biogenesis Changes on ROS Generation Induced by High Glucose

Objective To study the effect of PGC-1α on vascular endothelial cells mitochondrial biogenesis and the influence of biogenesis on glucose induced ROS production.Methods Primary culture of BAECs and HUVECs were divided into four groups,each group was cultured in normal glucose solution and in high glucose solution(concentration: 5,30 mmol / L),and the intracellular ROS concentration was detected.Results Respective covariance analysis for BAECs and HUVECs showed that,the difference were statistically significant among the different groups(P 0.01),different glucose level had significant impact on the concentration of ROS(P 0.05);analysis on BAECs showed that the ROS concentration of PGC-1 α transfected group on normal glucose level was significantly lower than that of the control group and empty plasmid group(P 0.01),on high glucose level was lower than that in the control group and the empty plasmid group(P 0.05);the ROS concentration of PGC-1 α siRNA transfected group on normal glucose level was significantly higher than that of the control group(P 0.01),no statistically significant difference in comparison with the empty plasmid group(P 0.05);analysis on the HUVECs showed that the ROS concentration of PGC-1 α infected group was lower than the empty virus Ad group and the control group on normal and high glucose level(P 0.01);the ROS concentration of PGC-1 α siRNA transfected group on normal glucose level was significantly higher than the control group(P 0.01),no significant difference between Ad group and empty virus group(P 0.05).Conclusion On high glucose level,more ROS is generated by vascular endothelial cells than on the normal glucose level,but after PGC-1α protein is over expressed,the intracellular ROS concentration is obviously decreased;otherwise the ROS concentration is significantly increased.