2001•Europe PMC (PubMed Central)Requires access
The experimental study on in vitro expansion of human umbilical cord blood hematopoietic stem/progenitor cells
Su Liping, Hao Fu-rong, Zhang Yongfang, Yanyan Bai, Jingcheng Miao, Zhang Zhan-ying, Lansheng Zhang
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Abstract
Objective To investigate the expansion effects of the cytokine combination (FL+TPO+SCF +IL-6) on purified CD34 + cells obtained from cord blood.Methods Cord blood CD34 + cells were purified with Mini-MACS and cultured in stroma-free liquid culture system, the expansion effect on CD34 + cells was assayed by indirect immunofluorescence staining, flow cytometry, colony-forming test.Results Four weeks later, the number of cells and CFU of group FL+TPO+SCF+IL-6 were increased significantly compared with that in the pre-cultured( P 0.05 ),there were also some proportional CD34 +, Thy-1 + cells. The number of CFU reduced in the se cond ,CFC and CD34 + cells disappeared in the 4th week.Conclusion The cytokine combination of FL+TPO+SCF +IL-6 can more effectively expand CD34 + cells compared with the classical cytokine combination of SCF+IL-6+IL-3+GM-CSF+EPO and is an optimized project.