Treatment of allergic rhinitis rats by blockade of GATA3 expression with absorbed interferon gamma

Objective To investigate the efficacy and mechanism of absorbed interferon gamma (IFN-γ) in treatment of allergic rhinitis(AR) in rats.MethodsOvalbumirl (OVA) and aluminum hydroxide were used to construct the AR model.24 AR rats were randomly divided into positive control group(group B,n=8),IFN-γ treatment group(group C,n=8) and beclomethasone dipropionate treatment group(group D,n=8).The negative control group was consisted of 8 normal rats.After the AR models built,50 μl PBS,1 μg IFN-γ and 3.5 μg beclomethasone dipropionate was respectively dropped in each nasal cavity of every rat in group B,group C and group D from day 31 to 38 once daily.The nasal mucosa were collected on day 39 in order to observe the pathologic changes,and the expression of GATA-3 were determined by immunohistochemistry SP.ResultsThe nasal mucosa of AR rats was obviously of congestion,edema and thickening with inflammatory cells infiltration mainly of eosinophils in group B.But the above mentioned changes were much more ameliorated in group C and D.GATA3 expression was showed decreased in the nasal tissue in group C and D,significantly increased in group B.ConclusionsThe curative effect of IFN-γ might be involved the mechanisms of restraining the Th2 reaction by blockade of GATA3 expression in the nasal tissue in AR rats.