ScholarLens Back to search
2011•Science and Technology of Food IndustryRequires access

Study on properties and inhibitions of peroxidase from Dioscorea Hemsleyi

Yanfang Zhang

Open publisher page 1 citations

Abstract

Peroxidase(POD)of Dioscorea Hemsleyi was determined spectrophotometrically at 470nm using guaiacol as substrate,and the effect of different temperature,pH,substrate concentration,enzyme concentration,inhibitor and activator on POD were studied.The results showed that the optimum pH and temperature of POD were pH6.0 and 60℃ respectively,and Km=0.08231mol/L,Vmax=541.518U/(g·min).The order of inhibitors effects on POD was as follows:VC L-CysNaHSO3citric acidEDTA,and the order of activators effects on POD was as follows:CuSO4FeCl3.

About this research paper

What this paper is about

Peroxidase(POD)of Dioscorea Hemsleyi was determined spectrophotometrically at 470nm using guaiacol as substrate,and the effect of different temperature,pH,substrate concentration,enzyme concentration,inhibitor and activator on POD were studied.The results showed that the optimum pH and temperature of POD were pH6.0 and 60℃ respectively,and Km=0.08231mol/L,Vmax=541.518U/(g·min).The order of inhibitors effects on POD was as follows:VC L-CysNaHSO3citric acidEDTA,and the order of activators effects on POD was as follows:CuSO4FeCl3.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Peroxidase(POD)of Dioscorea Hemsleyi was determined spectrophotometrically at 470nm using guaiacol as substrate,and the effect of different temperature,pH,substrate concentration,enzyme concentration,inhibitor and activator on POD were studied.The results showed that the optimum pH and temperature of POD were pH6.0 and 60℃ respectively,and Km=0.08231mol/L,Vmax=541.518U/(g·min).The order of inhibitors effects on POD was as follows:VC L-CysNaHSO3citric acidEDTA,and the order of activators effects on POD was as follows:CuSO4FeCl3.

Key concepts: Point of delivery, Guaiacol, Peroxidase, Chemistry, Dioscorea, Substrate (aquarium), Enzyme, Food science

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Study on properties and inhibitions of peroxidase from Dioscorea Hemsleyi — Research Paper | ScholarLens