Proliferation inhibition and apoptosis induction of colorectal adenoma cells by sulindac

Objective To investigate the influence of sulindac,a non-selective cyclooxygenase(COX)inhibitor,on the proliferation and apoptosis of colorectal cancer cell HT-29 and its mechanisms.Methods Cultured HT-29 cells were treated with sulindac.MTT assay and flow cytometry were used to measure the proliferation and apoptosis respectively.The laser scaning microscope(LSM)and the fluorescence microscope were used to observe apoptosis of the cells,and the flow cytometry(FCM)was used to analyze its influence on the cell apoptosis and the cell cycle.Results Sulindac inhibited the cells proliferation and induced apoptosis in a dose and time-dependent manner.With the TUNEL staining and observing under the fluorescence microscope we found that the apoptsis cell became brown.After the AnnexinV/PI staining,we observed with LSM that the membrane of apptosis cells became green,its nucleosidase became red or crocus.FCM showed that this medicine promoted apptosis of the cells,made the cells in the stage of G0/G1 significantly reduced.Conclusion Sulindac may inhibit the proliferation and induce apoptosis of colon cancer cell HT-29,and its mechanism probably relates to the progress of its prevention on cell cycle.