ScholarLens Back to search
2009•Carcinogenesis,Teratogenesis and MutagenesisRequires access

Roles of TRAIL in Vitamine Succinate-induced Apoptosis in Human Gastric Cancer Cells

Kun Wu

Open publisher page 1 citations

Abstract

BACKGROUND AND AIM:In order to investigate the roles of TRAIL in vitamin E succinate(VES)-induced apoptosis in human gastric cancer MKN28 cells.MATERIALS AND METHODS:The MKN28 huamn gastric cancer cells were cultured with different concentrations of VES.The MKN28 cells were divided into four groups:5,10,20 μg/ml VES and control groups.Apoptosis was assessed by DAPI staining,and TRAIL-receptor(DR4,DR5,DcR1 and DcR2)protein expressions induced by VES at different doses and different time points were measured by western blot.RESULTS:The results showed that VES obviously induced cells to undergo apoptosis.The expression of DR4 and DR5 were increased by VES at 5,10,20 μg/ml for 12 h;however the expression of DcR1 and DcR2 were reduced.DR4 and DR5 were increased by the hour,then reached the top level at 12 h.CONCLUSION:The data implicated that TRAIL pathway might be invovled in VES-induced apoptosis.

About this research paper

What this paper is about

BACKGROUND AND AIM:In order to investigate the roles of TRAIL in vitamin E succinate(VES)-induced apoptosis in human gastric cancer MKN28 cells.MATERIALS AND METHODS:The MKN28 huamn gastric cancer cells were cultured with different concentrations of VES.The MKN28 cells were divided into four groups:5,10,20 μg/ml VES and control groups.Apoptosis was assessed by DAPI staining,and TRAIL-receptor(DR4,DR5,DcR1 and DcR2)protein expressions induced by VES at different doses and different time points were measured by western blot.RESULTS:The results showed that VES obviously induced cells to undergo apoptosis.The expression of DR4 and DR5 were increased by VES at 5,10,20 μg/ml for 12 h;however the expression of DcR1 and DcR2 were reduced.DR4 and DR5 were increased by the hour,then reached the top level at 12 h.CONCLUSION:The data implicated that TRAIL pathway might be invovled in VES-induced apoptosis.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

BACKGROUND AND AIM:In order to investigate the roles of TRAIL in vitamin E succinate(VES)-induced apoptosis in human gastric cancer MKN28 cells.MATERIALS AND METHODS:The MKN28 huamn gastric cancer cells were cultured with different concentrations of VES.The MKN28 cells were divided into four groups:5,10,20 μg/ml VES and control groups.Apoptosis was assessed by DAPI staining,and TRAIL-receptor(DR4,DR5,DcR1 and DcR2)protein expressions induced by VES at different doses and different time points were measured by western blot.RESULTS:The results showed that VES obviously induced cells to undergo apoptosis.The expression of DR4 and DR5 were increased by VES at 5,10,20 μg/ml for 12 h;however the expression of DcR1 and DcR2 were reduced.DR4 and DR5 were increased by the hour,then reached the top level at 12 h.CONCLUSION:The data implicated that TRAIL pathway might be invovled in VES-induced apoptosis.

Key concepts: Apoptosis, DAPI, Western blot, Cancer, Cancer cell, Chemistry, Blot, Staining

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Roles of TRAIL in Vitamine Succinate-induced Apoptosis in Human Gastric Cancer Cells — Research Paper | ScholarLens