Different expression of JKTBP in prostate cancer cell lines and prostate tissue

Objective To observe the different expression of JKTBP,a new member of hnRNPs,in prostate cancer cell lines(LNCaP and DU145)and prostate tissue(BPH,androgen dependent prostate cancer as AD-PCa and androgen independent prostate cancer as AI-PCa).Methods To detect the expressions of JKTBP in two prostate cancer cell lines(LNCaP and DU145)by RT-PCR and Western blotting.At the same time,to detect the different expression of JKTBP in prostate tissue by immunohistochemisty.Results There was remarkable difference in the expressions of JKTBP between the LNCaP and DU145.Both mRNA and protein were detected with high level of expression in DU145 cell line,which is known as AI-PCa cell line.Moreover,JKTBP was high positive expression in AI-PCa tissue,and relative low positive expression in AD-PCa tissue.However,negative expression was detected in BPH tissue.Conclusions There is definitely different in the expression of the JKTBP between AI-PCa cell line or tissue and AD-PCa cell line,tissue or BPH tissue.The expression of JKTBP may be play an important role in the promotion of prostate cancer,and could be a valuable index in evaluating the promotion of prostate cancer.