The Effect of Tea-glycoprotein on the Surface Molecules Expression Level of Murine Bone Marrow Derived Dendritic Cells

The effects of tea-glycoprotein(TGP) on the surface molecules expression level of murine bone marrow derived dendritic cells were observed in ouv study.Dendritic cells(DCs) generated from Balb/c murine bone marrow cells were induced by rmGM-CSF and rmIL-4.TGP was added to cells on day 6 of culture for 24 h.Morphology development of DCs was observed by invented microscope.The cytotoxicity of TGP were tested by using MTT method.The cells were analyzed by flow cytometry(FCM) to determine the proportion of CD11c cells and the change of CD86 and MHC class II molecule.The results showed that TGP treated DCs displayed a mole matured morphology,with long protrusions,while untreated DCs displayed shorter protrusions than stimulated DCs.When the concentration was below 200 μg/mL,TGP showed no cytotoxixity.TGP treated immature DCs showed an enhanced cell-surface expression of CD86 and MHC II on CD11c gated DCs,and at the concentration(0.1～25 μg/mL) was in a dose-dependent up-regulation.TGP could induce maturation of murine dendritic cells.