2007•Xibei zhiwu xuebaoRequires access
Somatic Embryogenesis and Establishment of Plant Regeneration System of Eruca sativa Mill.
Tao Zhang
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Abstract
Cotyledon explants of E.sativa were cultured to induce somatic embryogenesis(SEM) and then the somatic embryos were developed into seedlings.The results revealed that E.sativa genotypes and plant growth regulation agents(PGRs) had different effects on somatic embryogenesis,and the best medium to induce somatic embryogenesis of E.sativa was Murashige and Skoog(MS) medium supplemented with 1.0 mg·L-1 2,4-D.Secondary somatic embryos were reproduced on MS medium containing 0.2 mg·L-1 2,4-D.Maturation of somatic embryos showed that the somatic embryos were well developed and could differentiate into mature embryos on N6 medium.The mature somatic embryos were germinated and 45.2% of them could develope into plantlets by culturing on 1/2MS medium containing 0.1 mg·L-1 IBA.