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Apoptosis-inducing Effect of HrsTRAIL on Hepatoma Cell Line HepG2

Feng Quan-lin

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Abstract

Objective To study the apoptosis-inducing effect and its significance of human recombination soluble TRAIL(HrsTRAIL) on hepatoma cell line HepG2.Methods HepG2 cells were treated with HrsTRAIL and then the viability of HepG2 cells was measured by microculture tetrazolium dye (MTT) assay and apoptosis index was demonstrated by fluorescence-activated cell sorting(FACS). Results HrsTRAIL had potent antitumor activity in a time- and dose-dependent manner.After co-incubations of the HepG2 cells in the presence of HrsTRAIL at a concentration of 1 000 ng/ml for 24 hours, the viability of HepG2 cells decreased to 45%and the apoptosis index reached to 51%.Conclusion HrsTRAIL can kill HepG2 cells by inducing apoptosis,so HrsTRAIL might be a potential new cytotoxic drug for PHC.

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What this paper is about

Objective To study the apoptosis-inducing effect and its significance of human recombination soluble TRAIL(HrsTRAIL) on hepatoma cell line HepG2.Methods HepG2 cells were treated with HrsTRAIL and then the viability of HepG2 cells was measured by microculture tetrazolium dye (MTT) assay and apoptosis index was demonstrated by fluorescence-activated cell sorting(FACS). Results HrsTRAIL had potent antitumor activity in a time- and dose-dependent manner.After co-incubations of the HepG2 cells in the presence of HrsTRAIL at a concentration of 1 000 ng/ml for 24 hours, the viability of HepG2 cells decreased to 45%and the apoptosis index reached to 51%.Conclusion HrsTRAIL can kill HepG2 cells by inducing apoptosis,so HrsTRAIL might be a potential new cytotoxic drug for PHC.

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Available abstract

Objective To study the apoptosis-inducing effect and its significance of human recombination soluble TRAIL(HrsTRAIL) on hepatoma cell line HepG2.Methods HepG2 cells were treated with HrsTRAIL and then the viability of HepG2 cells was measured by microculture tetrazolium dye (MTT) assay and apoptosis index was demonstrated by fluorescence-activated cell sorting(FACS). Results HrsTRAIL had potent antitumor activity in a time- and dose-dependent manner.After co-incubations of the HepG2 cells in the presence of HrsTRAIL at a concentration of 1 000 ng/ml for 24 hours, the viability of HepG2 cells decreased to 45%and the apoptosis index reached to 51%.Conclusion HrsTRAIL can kill HepG2 cells by inducing apoptosis,so HrsTRAIL might be a potential new cytotoxic drug for PHC.

Key concepts: Microculture, Apoptosis, Viability assay, Cell sorting, Chemistry, Cell culture, Cytotoxic T cell, Molecular biology

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Apoptosis-inducing Effect of HrsTRAIL on Hepatoma Cell Line HepG2 — Research Paper | ScholarLens