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Study on Optimization for ISSR-PCR Reaction System of Ptychomitrum using Orthogonal Design

Cao Tong

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Abstract

Objective: Develop the most suitable ISSR-PCR system for Ptychomitrum.Method: The orthogonal design was used to optimize inter-simple sequence repeat PCR(ISSR-PCR) amplification system on Ptychomitrum in five factors(Mg2+,dNTPs,primer,DNA template and Taq DNA polymerase) at four levels respectively.Result: The most suitable ISSR-PCR system for Ptychomitrum was established,namely 25μl reaction system containing 3.2mmol/L Mg2+,0.96mmol/L dNTPs,10ng DNA template,1.04μmol/L primer,and 1.5U Taq DNA polymerase.Using the optimized reaction,16 Ptychomitrum materials were easy amplified with primer 564.Conclusion:The result provided a standardizing program for the analysis of interspecies genetic diversity of Ptychomitrum.

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What this paper is about

Objective: Develop the most suitable ISSR-PCR system for Ptychomitrum.Method: The orthogonal design was used to optimize inter-simple sequence repeat PCR(ISSR-PCR) amplification system on Ptychomitrum in five factors(Mg2+,dNTPs,primer,DNA template and Taq DNA polymerase) at four levels respectively.Result: The most suitable ISSR-PCR system for Ptychomitrum was established,namely 25μl reaction system containing 3.2mmol/L Mg2+,0.96mmol/L dNTPs,10ng DNA template,1.04μmol/L primer,and 1.5U Taq DNA polymerase.Using the optimized reaction,16 Ptychomitrum materials were easy amplified with primer 564.Conclusion:The result provided a standardizing program for the analysis of interspecies genetic diversity of Ptychomitrum.

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Available abstract

Objective: Develop the most suitable ISSR-PCR system for Ptychomitrum.Method: The orthogonal design was used to optimize inter-simple sequence repeat PCR(ISSR-PCR) amplification system on Ptychomitrum in five factors(Mg2+,dNTPs,primer,DNA template and Taq DNA polymerase) at four levels respectively.Result: The most suitable ISSR-PCR system for Ptychomitrum was established,namely 25μl reaction system containing 3.2mmol/L Mg2+,0.96mmol/L dNTPs,10ng DNA template,1.04μmol/L primer,and 1.5U Taq DNA polymerase.Using the optimized reaction,16 Ptychomitrum materials were easy amplified with primer 564.Conclusion:The result provided a standardizing program for the analysis of interspecies genetic diversity of Ptychomitrum.

Key concepts: Primer (cosmetics), Polymerase chain reaction, DNA, Molecular biology, System optimization, Genetic diversity, Biology, Microsatellite

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Study on Optimization for ISSR-PCR Reaction System of Ptychomitrum using Orthogonal Design — Research Paper | ScholarLens