The study on the apoptosis mechanism of the Jurkat cell induced by cross linking anti-DR5 antibody-YM366EC

Objective:To detect the effect of cross-linking anti-DR5 YM366EC antibody on the Jurkat cells, and to elucidate the anti tumour mechanism of anti-DR5 antibody.Methods:Morphologic changes of Jurkat cells were observed by a microscope;cytotoxic and apoptotic effects of cross-linking anti-DR5 to Jurkat cells were detected by MTT and FCM assays.Bcl-2,Cyt-C,caspase3,Bax and caspase9 were detected with Western blot.Results:Chromatin aggregation,budding and apoptotic bodies were seen in Jurkat cells treated by cross-linking anti-DR5 antibody;viability of Jurkat cells was decreased and apoptosis increased obviously.Expression of Bcl-2,Cyt-C,caspase3,Bax and caspase9 were detected, and they were changed with time of cross-linking anti-DR5 antibody treated.Conclusion:Apoptosis of Jurkat cell can be induced by the cross-linking anti-DR5 antibody, and the apoptosis signal pathway may be relevant to Cyt-C,caspase3,Bax and caspase9.