Expression of TLR9 mRNA in BV2 cells after HCV stimulation

Objective To detected the expression of TLR9 after mouse microglia(BV2)were stimulated by hepatitis C virus(HCV),so as to explore the immune mechanisms against HCV through TLR9 in microglia.Methods BV2 cells were divided into 3 groups: HCV-positive serum group,normal serum group and blank control group.BV2 cells were collected at 4、12、20、36 hours after cultivation,and then the expression of TLR9 mRNA was detected on different times by real-time quantitative PCR(RT-qPCR).Results There was TLR9 mRNA expression in BV2 cells.The expression of TLR9 mRNA in HCV-positive serum group at different time was 3.50±0.33、2.03±0.10、2.05±0.12、2.02±0.14,respectively;they were higher than those in normal serum group(0.99±0.08、1.01±0.09、1.00±0.10、0.99±0.09)and blank control group(all in 1)(P0.01);and in HCV-positive serum group the expression of TLR9 mRNA was the highest at 4h(P0.01).Conclusions BV2 cells could express TLR9 mRNA,and the expression of TLR9 was significantly increased after HCV stimulation,TLR9 might be involved in immune process of BV2 against HCV.