2008•Biotechnology(Faisalabad)Requires access
Optimization of Major Factors for Agrobacterium-mediated Genetic Transformation of Callus of Wheat Mature Embryo
Ruiming Bi
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Abstract
Objective:Transformation efficiency of mature embryo of wheat mediated by Agrobacterium tumefacienens would be raised.Method:In this paper,common wheats HB341,SN2618 and GV3101/pBI121 strain of Agrobacterium tumefaciens were used as test material.Major factors for Agrobacterium-mediated genetic transformation of callus of wheat mature embryo viz.selection pressure of selection reagent,pre-cultivation duration of explant,Agrobacterium tumefaciens cell density and inoculation duration had been studied.Result:The optimum transformation conditions were 3 days pre-cultivation duration of explant,OD600=0.6 for Agrobacterium tumefaciens cell density,30 minutes for inoculation duration,40～60 mg l-1 kanamycin.Conclusion:This paper supplied gists for the establishment of fast and efficient genetic transformation system of mature embryo callus of wheat mediated by Agrobacterium tumefacienens.