SELECTION OF CULTURE CONDITIONS FOR LACCASE BIOSYNTHESIS WITH FLAMMULINA VELUTIPES

Induction on the laccase biosynthesis with Flammulina velutipes was enhanced with the higher DP carbon source and organic nitrogen source.Among experimented substrates,xylo-oligosaccharides and ammonia tartrate are the best carbon source and the best nitrogen source,respectively.The laccase activity yielded with starch could reached 93.9% as much as xylo-oligosaccharides and the result from organic nitrogen sources is satisfying,so starch and (soybean) powder was selected as carbon source and nitrogen source for Flammulina velutipes biosynthesizing laccase,(respectively).By enhancing the transfer in a liquid medium,shaking could improve growth rate and enhance laccase excretion of Flammulina velutipes.The laccase activity reached 308 IU/mL in the shaking culture,which was 83.3% more than that in the static culture.The laccase activity of Flammulina velutipes was improved remarkably as it was inoculated after activation and grew in a shaking fermentor,and it reached 1 749 IU/mL at 180 r/min for 6 days.