The effect of L-glutamine on intestinal injury following total hepatic inflow occlusion

Objectives:To explore the effect of L-glutamine(Gln) on intestinal injury following total hepatic inflow occlusion.Methods:Male Wistar rats were assigned randomly to three groups(n=40) :Group A,sham-operation;Group B,control group,rats were pretreated with 4 ml 0.9% saline intraperitonally twice per day on 5 consecutive days;Group C,rats were pretreated with Gln dissolved in 4 ml 0.9% saline intraperitoneally twice per day on 5 consecutive days.The rats in group B and C underwent total hepatic inflow occlusion for 35min by the pringle' s manoeuvre.Ten rats from each group were randomly sacrificed before occlusion and at 2,4,24h after reperfusion respectively.The levels of MDA,SOD,GSH in intestine tissue were measured.The levels of serum TNF-α and portal vein endotoxin were detected.Results:Compared with group A,the levels of GSH and SOD decreased after reperfusion(P0.05) ,but MDA,and TNF-α and endotoxin increased(P0.05) in group B.The levels of SOD in intestine in group C increased after reperfusion compared with group B(P0.05) ,that of MDA,TNF-α and endotoxin decreased(P0.05) .The levels of GSH in intestine increased at 2 and 4h after reperfusion in group C.Conclusion:Total hepatic inflow occlusion might result in the damage of intestinal barrier.Gln presupplement could alleviate the injury of gut after the pringle' s manoeuvre in some extent,furthermore reduced the translocation of gut-derived endotoxin and release of proinflammatory factors.