Antisense oligonucleotide targeting survivin inducing apoptosis of prostate cancer cell line PC-3 in vitro

Objective To investigate the effect of antisense oligonucleotide (ASODN) targeting survivin on the apoptosis and proliferation of prostate cancer cell line PC-3.Methods ASODN targeting survivin was designed and constructed.Cultured cells were divided into 6 groups:control group,liposomes group,sense oligonucleotide (SODN) group,200 nmol/L ASODN group,400 nmol/L ASODN group and 600 nmol/L ASODN group.After transfected for 24 h,the cultured cells were harvested to carry out the next tests.Cell morphological changes were observed under invert microscopy and transmission electron microscopy;Survivin protein expression was detected by immunohistochemical method and flow cytometry;Apoptosis index (AI) and proliferative index (PI) were also examined by flow cytometry;Inhibitory rate (IR) by ASODN was determined by the colorimetri MTT cell viability and proliferation assay.Results Abnormal morphological change of cells were observed in ASODN transfected groups.The expression of survivin in ASODN groups was significantly decreased as compared with that in the control,liposomes and SODN group;The AI in 200,400 and 600 nmol/L ASODN groups was (8.50±0.79)%,(13.78±0.56)%,(21.31±1.67)% respectively and significantly higher than other control groups (P 0.05),and that in the ASODN groups was significantly higher than that of any other groups.The PI in 200,400 and 600 nmol/L ASODN groups was (37.80±1.72)%,(25.10±2.18)% and (19.90± 0.97)% respectively,significantly lower than any other control groups (P0.05).There was no significance in PI and AI rate among the different control groups.The IR in 200,400 and 600 nmol/L ASODN groups was 60.0%,66.0% and 78.6% respectively.Conclusion The expression of survivin may be decreased in PC-3 cells after ASODN transfection.ASODN targeting survivin can induce PC-3 cell apoptosis and inhibit the proliferation of PC-3 cells.