Effects of Geraniin on Osteoclastic Bone-resorption Activity

Objective To evaluate the effects of geraniin(Ge) on the generation of osteoclasts(OCs) and its bone-resorption activity in vitro.Methods OCs isolated mechanically from long bones of SD rats aged one-day were plated in 48-well plates directly or on ivory bone slices in 24-well plates for culture together with different concentrations of Ge.TRAP staining and toluidine blue staining were used to evaluate the generation of OC and bone resorption on ivory slices.Results Ge decreased the total numbers of multinucleated TRAP-positive osteoclasts in cultures.Ge decreased the number of resorption pits on slices,total areas of resorption pits on slices as well as the average luminance of pits.Conclusion Ge can inhibit the generation of OCs and the bone-resorption activity of osteoclasts in vitro.