Gene Cloning of AtDREB2B and Constructtion of Its Plant Expression Vectors

[Objective] The research aimed to obtain the plant drought-resistant transcription factor and its expression vector,then the new plant strains of drought-resistant plants.[Method] Total RNA was obtained from dehydrated Arabidopsis.And the AtDREB2B special primers were used to amplify 1.2 kb fragment by RT-PCR.This 1.2 kb fragment was inserted into pBS-T and sequenced.[Result] Sequence analysis showed that the sequence cloned in this experiment had a similarity of 100% with AtDREB2B gene logged on GenBank.Furthermore the subcloned fragment was inserted into plant expression vectors pCAMBIA1301 and pBin438 b,which were expressed by rd29A promoter and repeated 35 S promoter regulated gene separately.[Conclusion] 2 plant expression vectors of AtDREB2B was constructed successfully.And a basic way for using Agrobacterium mediated AtDREB2B gene transformation was provided with the freezing-melting transformation of Agrobacterium GV3101.