An experimental study of the expression of proto-oncogenes during focal cerebral ischemia/reperfusion in Wistar rats

Objective For investigate the mechanism of proto oncogenes manipulating the cerebral ischemia in focal ischemia/reperfusion rats.Methods A model of the middle cerebral artery occlusion/reperfusion(MCAO/R) in Wistar rats was performed with the intraluminal filament occlusion.The rats brains were cut in the coronal planes at the caudoputamen as the templates.After performing the sham operation and MCAO for 1.5h followed by reperfusion 4h,24h and 72h respectively, the distributions and quantities of c fos and c jun, apoptosis and necrosis cells in the brain tissue were detected by immunohistochemical staining. Results Both apoptosis and necrosis coexist in focal cerebral ischemia/reperfusion rats model.The number of apoptotic cells increased markedly 4h after reperfusion of the MCA, and reached to a summit 24h after reperfusion. However, it began to decrease 72h after reperfusion(I/R). The distribution of apoptotic cells were detected in cerebral cortex, ischemic penumbra(IP) and hippocampus,etc. The protein experession of c fos and c jun are mainly localized in ischemic core,IP and cerebral cortex. The expression levels of c fos and c jun markedly increased 4h after reperfusion of the MCA, and began to gradually decrease in the period of 24h and 72h reperfusion( P 0 05).Conclusion Cerebral ischemia can induce the abnormal experession of c fos, c jun and generate large amounts of apoptotic cells. The mechanism of cerebral ischemia probably relates to the abnormal expression of c fos and c jun.