Hand-foot-mouth disease laboratory diagnosis results analysis of Chongzuo city in 2013

Objective To understand the pathogen characters of hand-foot-mouth disease( HFMD) and provide reference for laboratory diagnosis of HFMD. Methods A total of 432 samples including throat swabs,Herpes fluid,anal swab,from 428 patients all over Chongzuo city in 2013. Nucleic acids of 2 types of viruses of human enterovirus 71( EV71) and Coxasckie virus A16( CVA16) were detected by real time RT-PCR method both in original samples. Results In 432 samples from the patients,41 were EV71 positive,67 were CVA16,no specimen with combined infection of EV71 and CVA16 was detected. In the 428 HFMD cases,the EV71 positive rate was 9. 58 %( 41 /428),and the CVA16 positive rate was 15. 65 %( 67 /428). 13 cases of HFMD in severs cases,4 cases were EV71 infecton,1 case of CVA16. The proportion of male and female in the cases of HFMD was 1. 84: 1,no statistically significant difference( χ2= 0. 001,P 0. 05).No statistical differences was anal swab specimens of virus detection rate and the pharyngeal swab specimens( χ2= 1. 344,P 0. 05). Conclusions CVA16 was the dominant pathogen of the HFMD in Chongzuo city in2013,and the EV71 was main strains causing HFMD of severe cases.