Effect of Total Saponin of Dioscoreae Nipponicae Rhizoma on PI3K/AKT Signal Pathway by in rIL-1β Induced Fibroblast-Like Synoviocytes

Objective:To explore the effects of total saponin of Dioscoreae Nipponicae Rhizoma(RDN) on in vitro apoptosis of rIL-1β induced fibroblast-like synoviocytes(FLS),and further to investigate the PI3K/AKT signal mechanism.Method: FLS were primarily cultured.Model group: 10 μg · L-1 rIL-1β,RDN group: 10 μg · L-1 rIL-1β and 100 μg · L-1 RDN.Western blot method was applied to examine the expression of p-PI3K protein and p-AKT protein degradation in rIL-1β induced FLS stimulated with total saponin of RDN.Result: Compared to control group,expression of p-PI3K and p-AKT protein increased in model group,respectively 162.66±2.34 and 157.03±1.84(P0.05),total saponin of RDN inhibited rIL-1β induced phosphorylation of AKT and PI3K in FLS,respectively 67.84±1.24 and 71.66±1.20(P0.01).Conclusion: Total saponin of RDN can inhibit FLS PI3K/AKT signal pathway.Total saponin of RDN may provide a new therapeutic approach in treatment of gouty arthritis.