Protective Effect of DHM on 3-nitropropionic acid induced oxidative stress in PC12 cells

Objective To investigate the protective effect and the possible mechanism of dihydromyricetin(DHM) on injury induced by 3-nitropropionic acid in PC12 cells. Methods HD model was induced by addition of 3-NP into PC12 cells for 24h. PC12 cells were pretreated with different doses of DHM to explore the optimum concentration of the best protective effect. Cell viability, the level of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) were measured by MTT assay, the method of thiobarbituric acid reaction and the method of water-soluble tetrazolium salt reaction. Results MTT assay showed that 3-NP dose-dependently decreased cell viability and the 24 h LC50 for 3-NP in PC12 cells was 8 mmol / L. Cell viability was significantly increased by DHM at the concentration of 100 μmol / L and 10 μmol / L. Further, DHM treatment significantly downregulated. the level of MDA in PC12 cells increased by 3-NP(P 0.05) and also restored the activity of SOD in PC12 cells which was profoundly lowered by 3-NP(P 0.05).