Roles of endotoxin binding peptide in rat model of burn combined with endotoxemia

Objective To observe the protective effect of recombinant human endotoxin binding peptide (EBP) on a rat model of burn combined with endotoxemia. Methods A total of 78 rats of model of burn combined with endotoxemia were divided into three groups: model group ( n =36), treatment group ( n =36), and control group ( n =6). Rats in the model group were intraperitoneally injected with 1 mg/kg endotoxin (prepared with 1 ml saline) immediately after burn. After intraperitoneal injection of 1 mg/kg endotoxin (prepared with 0.5 ml saline), rats in the treatment group were intraperitoneally injected with EBP (prepared with 0.5 ml saline). Blood and liver and lung tissues of rats in the model and treatment groups were collected at 1, 3, 6, 12, 24, and 48 h after treatment. Rats in the control group were intraperitoneally injected with 1 ml saline after trichomadesis. Blood and liver and lung tissues of rats in the control group were collected for the total control. The changes of alanine aminotransferase (ALT), TNF α, and IL 6 contents in serum were determined by biochemical velocity analysis, ELISA, and light microscopy. The pathological changes of the liver and lung tissues were observed light microscopically and electron microscopically. Results The serum TNF α level of rats in the model group was significantly higher than that in the control at 1 h after injury ( P 0.01) and reached the peak value at 6 h. Serum TNF α level of rats at each time point in the treatment group was significantly lower than that in the model group ( P 0.01, P 0.05). Serum Il 6 level of rats in the model group was significantly higher than that in the control group at 1 h after injury ( P 0.01), and reached the peak at 12 h. The serum IL 6 levels during 3-48 h in the treatment group were significantly lower than those in the model group. The dynamic changes of ALT showed that the serum ALT level in the model group increased significantly, as compared with that in the normal control group ( P 0.01), but the serum ALT level in the treatment group decreases significantly, as compared with that in the model group ( P 0.01, P 0.05). Histopathological changes revealed attenuated degree of degeneration of hepatocytes, decreased inflammatory cell infiltration, and attenuated interstitial congestion and hemorrhage and inflammatory changes of alveoli in the lungs. Conclusion The recombinant human EBP may possess a biological activity and play a potential protective role in the rat model of burn combined with endotoxemia.