Regulation of 1 Alpha,25-dihydroxy Vitamin D_3 to IL-10、IL-12 in Children with Hashmoto's Disease

Interleukin-12、IL-10,as one of cytokines of type Th1,Th2 respectively,to analysis the tiny change of IL-12,IL-10 under intervention of 1α,25-dihydroxy vitamin D_3 at the level of monocytes in children with hashmoto's thyroiditis(HT),and to study the important regulative role of 1α,25-dihydroxy witamin D_3 for HT.Human peripheral blood monocytes(PBMCs) from 27 HT patients and 17 healthy children isolated from heparinized peripheral blood by density gradient centrifugation with Ficoll-Paque were isolated furtherly to get monocytes,cultured under the condition in the presence or absence of 1α,25(OH)_2D_3 respectively,then collected the culture supernatant in sterility.IL-10 and IL-12 were measured by enzyme-linked immunosorbent assay(ELISA)in the culture supernatant.Meanwhile serum was gotten from another part of the peripheral blood,then 25(OH)D_3 was extracted and estimated by radioimmunoassay(RIA).The results demonstrated the level of 25(OH)D_3 decreased strongly compared with healthy controls in HT,(21.85±5.73)ng/mL vs(27.56±7.46)ng/mL(P0.01)respectively,the level of IL-12 was significantly increased compared with healthy controls,(119.18±28.65)pg/mL vs(102.84±23.86)pg/mL(P0.01)respectively,while IL-10 decreased significently,(132.99±12.04)pg/mL vs(171.41±35.72)pg/mL(P0.01) respectively.And most important,in the presence of 1α,25(OH)_2D_3,IL-12 was dramatically down-regulated in HT,(98.57±11.98)pg/mL(P0.01),however,IL-10 both in HT and in control group widespreadly increased,(184.15±35.34)pg/mL(P0.01),(223.77±53.36)pg/mL(P0.01) respectively,so the patients with HT had a higher ratio of ρ(IL-10)/ρ(IL-12)in response to 1,25(OH)_2D_3.The level of 25(OH)D_3 was deficient seriously in HT,1α,25(OH)_2D_3 took part in the accommodation process of cytokines secretion from monocytes;it could inhibit the increased Th1 cytokines IL-12,increase IL-10,so it could correct the imbalance of Th1/Th2 cytokines of HT.