2006•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Study on infection of nondividing myeloma cells by lentivirus vector
Xiaoxing Cheng
Open publisher page 0 citations
Abstract
Objective To study the efficient and stable transduction of nondividing myeloma cells J558L by lentivirus vector. Methods The lentivirus vector to express EGFP (Enhance green fluorescence protein) and NeoR (Neomycin resistance gene) were constructed respectively. The expression of EGFP was observed by fluorescence microscope and flow cytometer. The J558L cells affected by lentivirus were screened by G418. Results The titer of lentivirus was 6×105 IU (Infected Unit)/mL, transduction efficiency ranged from 6~11%. The J558L cells affected by lentivirus could express NeoR gene stably. Conclusion The nondividing J558L cell could be transducted efficiently by lentivirus vectors. Lentivirus is an efficient vector of gene therapy on hematological system disease.