Pollen Germination Characteristics and Conditions Selecting for Three Plum Varieties
Xue Xiao-mi
Abstract
Xue Xiao-mi
Abstract
In order to improve the efficiency of liquid pollination in plum,the pollens of 3plum varieties were used to study the effects of different mediums and different culture conditions on pollen germination rate and pollen tube growth.The results showed that the pollen germination rate was first increased and then decreased with the increase of sucrose concentration,boric acid concentration and incubation temperature,and the optimal medium was 10%sucrose+100μL·L-1 boric acid,the most suitable culture temperature was 25℃.The plum's pollen germinated sooner,the germination rate was 28%for 2hlater and reached the maximum value for 4h later,then the germination rate of pollen was not increased with the time extension.Pollen germination rate and pollen tube length of 3plum varieties were determined under the selecting condition,pollen germination rate of Jinhong was 42.89% which was the highest among them,and pollen tube length of Jinyan was 35.97μm which was the longest among them,the pollen germination percentage and pollen tube length of Zaomeili were the lowest among three varieties.
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In order to improve the efficiency of liquid pollination in plum,the pollens of 3plum varieties were used to study the effects of different mediums and different culture conditions on pollen germination rate and pollen tube growth.The results showed that the pollen germination rate was first increased and then decreased with the increase of sucrose concentration,boric acid concentration and incubation temperature,and the optimal medium was 10%sucrose+100μL·L-1 boric acid,the most suitable culture temperature was 25℃.The plum's pollen germinated sooner,the germination rate was 28%for 2hlater and reached the maximum value for 4h later,then the germination rate of pollen was not increased with the time extension.Pollen germination rate and pollen tube length of 3plum varieties were determined under the selecting condition,pollen germination rate of Jinhong was 42.89% which was the highest among them,and pollen tube length of Jinyan was 35.97μm which was the longest among them,the pollen germination percentage and pollen tube length of Zaomeili were the lowest among three varieties.
Key concepts: Germination, Pollen, Pollen tube, Boric acid, Pollination, Sucrose, Biology, Horticulture