2006•PubMedRequires access
[Effects of sodium arsenite on catalase in human keratinocytes].
Xiance Sun, Fengyuan Piao, Yi Wang, Xu YuanYuan, Xin Li, Bing Li, Yaping Jin, Guifan Sun
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Abstract
OBJECTIVE: To evaluate the effects of sodium arsenite on the activity, the mRNA and the protein expression of CAT in human keratinocyte cell line (HaCaT). METHODS: The activity of catalase (CAT) was detected by ultraviolet direct velocity assay. RT-PCR was used to detect the mRNA expression of CAT and Western blotting was conducted to detect the protein expression of CAT. RESULTS: If the cells were treated with higher than 5.0 micromol/L sodium arsenite, the activity, mRNA and protein expression of CAT were decreased significantly and in a dosage dependent fashion (P < 0.05). CONCLUSION: CAT is inhibited by sodium arsenite in the transcription, translation and activity levels.