Establishment of an animal model of slow tramit constipation and the investigation of its mechanism

Objective:To establish an animal model of slow transit constipation and the pathobiological changes in interstitial cell of Cajal in colon. Methods:The mouse model was established by subcutaneous administration of morphine. Fecal weight was recorded daily. Transit functions of intestinal movement were examined by activated charcoal suspension pushing test and the changes of interstitial cell of Cajal were observed by immunohistochemical methods. Results:Compared with the controlled mice, there was a significant decrease in fecal weight daily(P0.01), intestinal transit ratio and number of interstitial cell of Cajal in colon tissue were significant decreased(P0.01)in mice with morphine. Conclusions:The animal model of slow intestinal transit movement induced by morphine conforms with clinical characteristic of slow transit constipation,endogenous opium peptide may leads to reduce of interstitial cell of Cajal, and at last maybe result in slowing motility of intestine.