Researches on cumulation of protoporphyrin IX in SW480 cells induced by DFO and 5-ALA

[Objective] To explore the characteristics of cumulation of protoporphyrin Ⅸ in SW480 cells induced by DFO and 5-ALA. [Method] SW480 cells were respectively cultivated in 4 groups, Group A, B, C, D, using culture solution, RPMI1640 with DFO 0.1 mmol/mL, 0.1 mmol/mL DFO + 2 mmol/mL 5-ALA, 5-ALA 2 mmol/mL, without DFO, 5-ALA for 10 hr. Fluorescence distribution and intensity of protoporphyrin Ⅸ in SW480 cells were observed under fluorescence microscope, the content of protoporphyrin Ⅸ in SW480 cells were quantitatively analyzed by high performance liquid chromatography. [Results] Different intensities of red fluorescence can be observed in SW480 from Group B and Group C. The fluorescence intensity of protoporphyrin Ⅸ from Group B is significantly higher than that from Group C. The count of high intensive fluorescence cells are respectively (57.67±2.49)% and (20.07±2.05)%, with significant difference (P 0.001); the content of protoporphyrin Ⅸin SW480 cells from 4 groups are different, Group A Group D Group C Group B. [Conclusion] It could efficiently increase cumulation of protoporphyrin IX in SW480 cells induced by DFO and 5-ALA.