The Effect of XZL on Apoptosis of HepG_2 Cells and Expression of P_(53) and nm_(23)-H_1

Objective:To observe the apoptosis of hepatoma carcinoma cell HepG2-in vitro-induced by Xiaozhiling injection(XZL),and discuss its mechanism of action.Methods:HepG2 cells were cultured in vitro with different concentrations of XZL(0.5、1、2、4、8、16g/L),and the cell inhibition rates were detected by cytotoxicity test,then Western blotting was used to detect the changes of correlated apoptosis protein P53 and nm23-H1.Results:(1)XZL significantly restrained the growth of HepG2 cells,presenting time and concentration dependent,(2)compared with the control group,the expression of P53in the XZL group was markedly higher(P0.05),compared with the 5-Fu group,there are no significant difference in the expression of P53(P0.05),compared with the co-action group,the expression of P53 in the XZL group significantly lower(P0.05),but the difference of expression of nm23-H1 between each group were not significant(P0.05).Conclusion:XZL can inhibit HepG2 cells multiplication,and induce the apoptosis of HepG2 cells by increasing the expression of P53.