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2007•Chinese Journal of Birth Health & HeredityRequires access

Application of the single blastomere PCR for preimplantation genetic diagnosis.

Yongjian Chen

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Abstract

Objectives:This study is to set up a technique of single blastomere PCR,and to provide a basis for preimplantation genetic diagnosis.Methods:We amplified 103 blastomeres by PCR,including 30 blastomeres for KG8,33 blastomeres for D16S291 and 40 blastomeres for D16S423.Results:The amplification success rate of the single blastomere for KG8,D16S291 and D16S423 were 86.7%,87.9% and 87.5%,respectively.Conclusion:Single blastomere PCR is stable and reliable,and it can be used for preimplantation genetic diagnosis.

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What this paper is about

Objectives:This study is to set up a technique of single blastomere PCR,and to provide a basis for preimplantation genetic diagnosis.Methods:We amplified 103 blastomeres by PCR,including 30 blastomeres for KG8,33 blastomeres for D16S291 and 40 blastomeres for D16S423.Results:The amplification success rate of the single blastomere for KG8,D16S291 and D16S423 were 86.7%,87.9% and 87.5%,respectively.Conclusion:Single blastomere PCR is stable and reliable,and it can be used for preimplantation genetic diagnosis.

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Available abstract

Objectives:This study is to set up a technique of single blastomere PCR,and to provide a basis for preimplantation genetic diagnosis.Methods:We amplified 103 blastomeres by PCR,including 30 blastomeres for KG8,33 blastomeres for D16S291 and 40 blastomeres for D16S423.Results:The amplification success rate of the single blastomere for KG8,D16S291 and D16S423 were 86.7%,87.9% and 87.5%,respectively.Conclusion:Single blastomere PCR is stable and reliable,and it can be used for preimplantation genetic diagnosis.

Key concepts: Blastomere, Preimplantation genetic diagnosis, Biology, Embryo, Andrology, Genetics, Medicine, Embryogenesis

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