Asymmetric siRNA targeting VEGF inhibits proliferation of breast cancer MCF-7 cells

Objective: To investigate the inhibitiory effect of siRNA targeting vascular endothelial growth factor(VEGF) gene on proliferation of breast cancer MCF-7 cells.Methods: Small interfering RNA(siRNA) targeting VEGF,including 2 siRNAs(siRNA21 /21,siRNA23 /23) and 2 asymmetric siRNAs(aiRNA)(aiRNA19 /21,aiRNA21 /23) were designed and synthesized.The siRNA was transfected into MCF-7 cells,and cell proliferation was detected by MTT method,and the apoptosis was analyzed by flow cytometry.VEGF protein and mRNA expression levels of MCF-7 cells were detected by ELISA and RT-PCR.Results: Compared with the control group,the 4 siRNAs effectively inhibited VEGF mRNA expression([71.4 ±5.01]%,[40.0 ±3.11]%,[37.2 ±2.79]%,[11.1 ±0.99]% vs[2.4 ±0.11]%,P 0.05),and the proliferation of MCF-7 cells([44.7 ± 5.38]%,[38.5 ± 5.67]%,[33.6 ± 2.18]%,[33.1 ± 3.18]%,[2.2 ± 0.28]%,P 0.05),with aiRNA21 /23 of the best RNA interference effect.Compared with the control group,the apoptotic rate was significantly increased([49.9 ± 4.02]% vs [4.7 ± 0.91]%,P 0.05).Conclusion: siRNA targeting VEGF can inhibit proliferation and induce apoptosis of MCF-7 cells,with asymmetric siRNA showing best effects.