Effects of alendronate on the proliferation,secretion and osteogenesis function of osteoblast

Objective To study the effects of alendronate on the function of osteoblasts and to explore the possibility of alendronaete in the prevetion and treatment of prosthesis aseptic loosening.Methods Using the method of in vitro cell culture,the influence of Alendronate at concentrations of 1×10 -11 mol/L,1×10 -9 mol/L,1×10 -7 mol/L,and 1×10 -5 mol/L on the osteoblastic function (proliferation,ALP activity,osteocalcin production and bone formation) was detected.Results Alendronate had no inhitory effects on osteoblastic proliferation except at a higher concentration (1×10 -5 mol/L),but increased ALP activity and osteocalcin production at concentrations of 1×10 -7 mol/L,1×10 -5 mol/L;It had a biphasic effect on osteoblastic bone-formation,being stimulatory at lower concentrations (1×10 -11 mol/L,1×10 -9 mol/L),and inhibitory at 1×10 -5 mol/L.Conclusion The effects of alendronate on the function of osteoblasts are associated with locally different concenteations.Lower concentration of alendronate stimulated local osteogenesis.It might be used as an effectine way for preventing prothesis aseptic loosening.