Development of cerebral venous sinus thrombosis animal model by temporary clipping and promoting coagulation

Objective To develop an animal model of cerebral venous sinus thrombosis(CVST) by temporary occlusion and promoting coagulation.Methods Thirty New Zealand white rabbits were randomly divided into three groups:temporarily clipped superior sagittal sinus group(n=10),ligating venous sinus control group(n=10) and sham control group(n=10).The rabbits of temporarily clipped superior sagittal sinus group were clipped of the superior sagittal sinus and injected with suspension of cephalin kaolin and cephalin.The rabbits of ligating venous sinus control group were ligated of the superior sagittal sinus.The rabbits of the sham control group were without operation.During experiment,the arterial blood pressure,heart rate and respiration were monitored.After experiment the water content of the brain tissue and blood flow speed of venous sinus were determined.Results The superior sagittal sinus of temporarily clipped superior sagittal sinus group was infarcted completely,and the blood flow speed was zero 5 hours after experiment.The arterial blood pressure,heart rate and respiration of the temporarily clipped superior sagittal sinus group were in the normal range 1 hour after experiment.The blood flow speed of the basal vein and water content of the brain tissue in the temporarily clipped superior sagittal sinus group and ligating venous sinus control group were significantly higher than those in the sham control group(P0.01),there were no statistically significant difference between the temporarily clipped superior sagittal sinus group and ligating venous sinus control group(P0.05).Conclusion The animal model by temporary clipping and promoting coagulation resemble clinical CVST with good reproducibility and stability,thus being appropriate for the experimental research of CVST.