Comparison of Anesthetic Effect and Application of Chloral Hydrate and Urethane and Their Equal Mixture on SD Rats

Objective:To compare the anesthetic effectiveness of Chloral hydrate,urethane and their 1:1 mixture on SD rats and test 1:1 mixture on the SD rat model making.Methods:Administration of different concentrations and doses of chloral hydrate,urethane and their 1:1 mixture anesthetized experiments to compare the anesthetic onset timespan,duration and mortality.Further the same doses of 1:1 mixture was used on the SD rat model making,to find the difference between the model rats and non-model rats.Results:Mixture of chloral hydrate and urethane anesthetized rats with the onset time of 2.5 ± 1.5 minutes,with no difference with the single water to-gether chloral(P 0.05),but faster onset time than single urethane(P 0.05);its duration 108.9 ± 4.4 minutes,longer than that of single water together chloral and urethane(P 0.01);its anesthesia mortality rates is lower than that of the single combined chloral of water,and its total mortality rate is also lower than that of the single use of water combined with chloral,or single use of urethane.In model group,anesthesia onset time 2.9 ± 1.6 minutes,duration 108.9 ± 4.4 minutes,zero anesthesia mortality,total mortality was 2.5%;there were no difference between the model group and the 1:1 mixture non-model group.Conclusions:1:1 mixture of chloral hydrate and urethane shows outstanding anesthetic effect in the initiation with negligible mortality.It's applicable to the animal surgery or model progress with the duration about 2 hours.