Effects of Short Hairpin RNA-Inhibited Survivin Gene on Jurkat Cell Apoptosis and Chemotherapy Sensibility

Objective To explore the effects of short hairpin RNA(shRNA)-inhibited survivin gene expression on apoptosis and the sensibility to chemotherapeutic drugs of human T lymphoblastic leukemia cell line Jurkat cell by transfected a shRNA against the expression of survivin gene.Methods The shRNA-Survivin vector was constructed and transfected into Jurkat cells by electroporation,the stable transfected cell line of changes in shRNA-Survivin recombinant plasmid was received by G418-selecting.The expression of Survivin mRNA in transfected cells was determined by reverse transcription-polymerase chain reaction.Treating the transfected cell line with vincristine(VCR) and daunorubicin(DNR),methyl thiazolyl tetrazolium assay was performed to evaluate the proliferation and flow cytometry labeled with propidium iodide was used to measure the cells apoptosis incidence.Results The recombinant plasmid was successfully constructed and the expression of Survivin mRNA significantly decreased in Survivin-shRNA transfected cells,compared with those in control-shRNA group and control group(Pa0.05).The stable transfected cells had higher sensitivity to VCR and DNR than those in control-shRNA group and control group(Pa0.05).Conclusions Survivin gene is very important for the growth of Jurkat cells,transfecting the shRNA-Survivin recombinant plasmid in Jurkat cell line can inhibit the expression of Survivin gene and increase the sensibility of apoptosis to chemotherapeutic drugs.