Tea Polyphenols Reduces Intercellular Adhesion Molecular-1 Expression in Plasma and Lung Injury Following Intestinal Ischemia/Reperfusion in Rats

Objective To investigate the effect of tea polyphenols on intercellular adhesion molecular-1(ICAM-1)expression in plasma and the lung tissue following intestinal ischemia/reperfusion(I/R)in rats.Methods 32 SD rats were randomly divided into 4 groups(n=8).①Group I/R in which rats were subjected to 1 hour of occlusion of the superior mesenteric artery(SMA),followed by 2 hours of reperfusion.②Early treatment group(P1),rats were subjected to the same procedure as group I/R with the additional administration of tea polyphenols beginning 10 minutes before ischemia with 10mg/kg loading dose,followed by continuous infusion at 10mg/(kg.h).③Treatment group(P2),rats were subjected identical insult as in group I/R with the administration of tea polyphenols started 10minutes before reperfusion with 10mg/kg loading dose,followed by continuous infusion at 10mg/(kg·h).④ Sham-operation group,rats were subjected to only.The ICAM-1 in plasma was assessed by ELISA.The ICAM-1 expression in pulmonary endothelium was assessed by histochemical staining.Results ICAM-1 in plasma and expression in lung tissue was increased significantly in group I/R and was decreased significantly by tea polyphenols.All the increase was less in quantity in the early treatment group of tea polyphenols than the other treatment group.Conclusion ICAM-1 plays an important role in lung injury after intestinal I/R.The early treatment of tea polyphenols before intestinal I/R may be more beneficial by reducing ICAM-1 expression in lung injury.