Molecular Cloning and Sequence Analysis of Chicken IFN-γ Gene

A pair of primers specific to ChIFN-γ gene were designed and synthesized according to the known sequences from the GenBank,and were used to amplify ChIFN-γ cDNA by RT-PCR from total RNA of chicken spleen cells induced by ConA.RT-PCR product was cloned into pMD18-T vector.The recombinant plasmid was identifled by digestion with restriction endonucleases and the nucleotide sequence was determined.The ChIFN-γ gene was 500 bp in length,with a 495 bp open reading frame encoding a polypeptide of 164 amino acids,the sequence homologous rate of ChIFN-γ and that from GenBank was 99.6%.