2007•Zhongguo xiandai yixue/Zhongguo xiandai yixue zazhiRequires access
Changes in extracellular-signal regulated protein kinase expression between normal and apoptotic lens epithelial cells cultured
Songbai Jia
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Abstract
[Objective] To use UVB-irridatiaon to cause apoptosis of cultured human lens epithelial cells(LECs),and to observe the changes in extracellular-signal regulated protein kinase(ERK) expression between normal and apoptotic LECs.[Methods] Cultured LECs were involved in this study.The test groups were irridated with UVB.And then the test and control groups were cultured for 12 hours.The expression of ERK of all the groups was detected using Western Blot method.[Results] The cells of the test group was half of that of the control group,but the expressions of ERK in the two groups were the same.Thus the expressions of ERK in the test groups were more than that in the control groups.[Conclusion] The UVB-irridatiaon can cause apoptosis of cultured LECs,when the expressions of ERK of the LECs increase.This study is useful to further research of ERK in formation of cataract and posterior capsular opacification.