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2011•Acta Academiae Medicinae Qingdao UniversitatisRequires access

EFFECTS OF ELECTROACUPUNCTURE ON DIFFERENTIATION OF NEURAL STEM CELLS OF RATS WITH CEREBRAL ISCHEMIA

Rui J

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Abstract

Objective To study the effects of electroacupuncture on differentiation of neural stem cells of rats with cerebral ischemia. Methods A model of middle cerebral artery occlusion(MCAO) in rats was created,which were randomly divided into electroacupuncture(EA) group and model group.The rats in EA group were punctured on acupoints of Bai Hui and Feng Fu,and those in model group were offered no punctures.The expressions of 5-bromodeoxyuridine/neun-specific enolase(BrdU/NSE) and 5-bromodeoxyuridine/glial fibrillary acidic protein(BrdU/GFAP) were detected with immunofluorescence double labeling method. Results The expressions of BrdU/NSE between the two groups(F=1 069.23,P0.01),and that of BrdU/NSE within the group at different time were significant(F=1.90,355.51.21;P0.01).The expressions of BrdU/GFAP between the two groups were different(F=28 100.21,P0.01),and that within one group at different time were also significant difference(F=6.50,90.18;P0.01). Conclusion Electroacupuncture can promote the expressions of NSE and GFAP in rats with cerebral ischemia.

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What this paper is about

Objective To study the effects of electroacupuncture on differentiation of neural stem cells of rats with cerebral ischemia. Methods A model of middle cerebral artery occlusion(MCAO) in rats was created,which were randomly divided into electroacupuncture(EA) group and model group.The rats in EA group were punctured on acupoints of Bai Hui and Feng Fu,and those in model group were offered no punctures.The expressions of 5-bromodeoxyuridine/neun-specific enolase(BrdU/NSE) and 5-bromodeoxyuridine/glial fibrillary acidic protein(BrdU/GFAP) were detected with immunofluorescence double labeling method. Results The expressions of BrdU/NSE between the two groups(F=1 069.23,P0.01),and that of BrdU/NSE within the group at different time were significant(F=1.90,355.51.21;P0.01).The expressions of BrdU/GFAP between the two groups were different(F=28 100.21,P0.01),and that within one group at different time were also significant difference(F=6.50,90.18;P0.01). Conclusion Electroacupuncture can promote the expressions of NSE and GFAP in rats with cerebral ischemia.

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Available abstract

Objective To study the effects of electroacupuncture on differentiation of neural stem cells of rats with cerebral ischemia. Methods A model of middle cerebral artery occlusion(MCAO) in rats was created,which were randomly divided into electroacupuncture(EA) group and model group.The rats in EA group were punctured on acupoints of Bai Hui and Feng Fu,and those in model group were offered no punctures.The expressions of 5-bromodeoxyuridine/neun-specific enolase(BrdU/NSE) and 5-bromodeoxyuridine/glial fibrillary acidic protein(BrdU/GFAP) were detected with immunofluorescence double labeling method. Results The expressions of BrdU/NSE between the two groups(F=1 069.23,P0.01),and that of BrdU/NSE within the group at different time were significant(F=1.90,355.51.21;P0.01).The expressions of BrdU/GFAP between the two groups were different(F=28 100.21,P0.01),and that within one group at different time were also significant difference(F=6.50,90.18;P0.01). Conclusion Electroacupuncture can promote the expressions of NSE and GFAP in rats with cerebral ischemia.

Key concepts: Electroacupuncture, NeuN, Glial fibrillary acidic protein, Bromodeoxyuridine, Ischemia, Neural stem cell, Immunohistochemistry, Medicine

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EFFECTS OF ELECTROACUPUNCTURE ON DIFFERENTIATION OF NEURAL STEM CELLS OF RATS WITH CEREBRAL ISCHEMIA — Research Paper | ScholarLens