Immunophenotype analysis of low-risk myelodysplastic syndrome

Objective To investigate the clinical significance of the classification,diagnosis and differential diagnosis of myelodysplastic syndromes(MDS).Methods A panel of monoclonal antibodies and the flow cytometry using CD45/SCC gating strategies were employed for the immunophenotyping on the bone marrow nucleated cells from 27 patients with low-risk MDS,control group and patients with AA as comparisons.Results The CD34+ expressions of low-risk MDS patients were increased.Immunophenotype of multiple protocell,matured granulocyte,monocyte expressed abnormally and were multiple representation.Conclusions Detecting abnormal bone marrow myeloblasts immunophenotype of MDS patients,is conducive to MDS diagnosis,differential diagnosis;flow cytometry is able to detect small changes,more sensitive than cytogenetics.