Effects of Recombinant Human Interleukin-1α and 1,25-(OH)2D3 on the Gene Expression of RANKL and OPG in HPDLFs

Objective: To evaluate the effects of rhIL-1α and 1,25-(OH)2D3 on the expression of RANKL and OPG in HPDLFs and to discuss the change of ratio of RANKL/OPG.Methods: HPDLFs were treated with 10ng/ml rhIL-1α,1×10-8mol/L 1,25-(OH)2D3 or 10ng/ml rhIL-1α plus 1×10-8mol/L 1,25-(OH)2D3 for 48 hours.The expression of RANKL and OPG were measured by FQ-RT-PCR.Results: RhIL-1α and 1,25(OH)2D3 influenced the expression of RANKL and OPG in HPDLFs.The expression of RANKL and OPG were increased with rhIL-1α(P0.05).Moreover,1,25(OH)2D3 up regulated the expression of RANKL and decreased the expression of OPG,so the ratio of RANKL/OPG was increased too(P0.05).There was cumulative effect on regulating the expression of RANKLwith the synergistic use of rhIL-1αand 1,25(OH)2D3,but there was no cumulative effect on the ratio of RANKL/OPG.Conclusion: RhIL-1α and 1,25-(OH)2D3 act through RANKL-OPG pathway to regulate the alveolar bone remolding.There was additive effect on the expression of RANKL with combination use of rhIL-1α and 1,25-(OH)2D3,but there was no additive effect or synergistic effect on the ratio of RANKL/OPG.