The study of detecting the serum level of anti-C1q antibody with ELISA

Objective To study the method of detecting the serum level of anti-C1q antibody.Methods Develop the procedure of detecting the serum level of anti-C1q antibody with ELISA.And among the anti-C1q antibody ELISA detection system,the concentration of the coated antigen and the enzyme labeled antibody is determined by the checkerboard titration technique.Finally sera from 92 healthy people were tested by the anti-C1q antibody ELISA detection system to decide the reference value.Results The concentration of the coated antigen is 5μg/ml,and the concentration of the enzyme labeled antibody is 1:50000.And coefficient of variability percent(CV%) of intro-batch and inter-batch reproducibility test was less than 15 %.According the result of the 92 people,the reference value is determined.(0-30.55 U/ml)Conclusion The specificity of the anti-C1q antibody ELISA detection system was approved nice by detecting small amounts of sera.And the procedure is not complicated.The anti-C1q antibody ELISA detection system is good for the serum level of anti-C1q antibody.