Determination of Six Constituents in Scutellaria Baicalensis Georgi by HPLC

OBJECTIVE To develop an HPLC method for determination of six indicative components(baicalin,wogonoside,baicalein,wogonin,chrysin,oroxylin-A) in Scutellaria baicalensis Georgi.METHODS Agilent ZORBAX SB-C18(250 mm×4.6 mm,5 μm)was adopted;the mobile phase was acetonitrile(A)-0.3% phosphoric acid-triethylamine(B) with gradient elution,and the detection wavelength was set at 275 nm,and flow rate was 1.0 mL?min?1 with the column temperature of 30 ℃.RESULTS The content of six elements in Scutellaria baicalensis Georgi was stable.The method had a good linearity in the ranges of 0.416 7?4.167 μg(r=0.999 2) for baicalin,0.078 15?0.781 5 μg(r=0.999 5) for wogonoside,0.053 21?0.532 1 μg(r=0.999 3) for baicalein,0.016 36?0.163 6 μg(r=0.999 3) for wogonin,0.001 160?0.011 60 μg(r=0.999 1) for chrysin,0.010 46?0.104 6 μg(r=0.999 4) for oroxylin-A.The average recoveris(n=5) were 99.4%,100.1%,99.9%,100.2%,100.4%,100.1%,respectively with RSDs of the recoveries were 1.9%,2.2%,2.3%,1.6%,2.2%,2.1%,respectively.CONCLUSION This method is acurate,simple and practical,and may be use to control quality of Scutellaria baicalensis Georgi.