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Effect of antisense oligonucleotide on expression of NR2B in hippocampal CAl following transient forebrain ischemia

Da Wei Teng

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Abstract

Objective: To study the effect of antisense oligonucleotide on NMDA receptor 2B (ANR2B) in hippocampal CA1 sub-field of rat following transient forebrain ischemia, so as to provide theoretical and morphologic basis for prevention and clinical treatment of cerebrovascular diseases, as well as for manufacturing and developing new drugs aimed at NMDAR subunits mRNA. Methods: ANR2B was designed, screened and synthesized at first. NMDA receptor 2B (SNR2B) was injected into the ischemia rat CA1 subfield before operating. Serial cryo-sections of brain from rats were examined under microscope after ABC immunohis-tochemistrystaining. Results: NR2B expression was obviously decreased (P0. 01) after ANR2B injection in CA1 subfield, but SNR2B. NS injection did not change NR2B expression. Conclusion: Expression of NR2B can be decreased in vivo by the antisense oligonucleotide technique.

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Objective: To study the effect of antisense oligonucleotide on NMDA receptor 2B (ANR2B) in hippocampal CA1 sub-field of rat following transient forebrain ischemia, so as to provide theoretical and morphologic basis for prevention and clinical treatment of cerebrovascular diseases, as well as for manufacturing and developing new drugs aimed at NMDAR subunits mRNA. Methods: ANR2B was designed, screened and synthesized at first. NMDA receptor 2B (SNR2B) was injected into the ischemia rat CA1 subfield before operating. Serial cryo-sections of brain from rats were examined under microscope after ABC immunohis-tochemistrystaining. Results: NR2B expression was obviously decreased (P0. 01) after ANR2B injection in CA1 subfield, but SNR2B. NS injection did not change NR2B expression. Conclusion: Expression of NR2B can be decreased in vivo by the antisense oligonucleotide technique.

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Available abstract

Objective: To study the effect of antisense oligonucleotide on NMDA receptor 2B (ANR2B) in hippocampal CA1 sub-field of rat following transient forebrain ischemia, so as to provide theoretical and morphologic basis for prevention and clinical treatment of cerebrovascular diseases, as well as for manufacturing and developing new drugs aimed at NMDAR subunits mRNA. Methods: ANR2B was designed, screened and synthesized at first. NMDA receptor 2B (SNR2B) was injected into the ischemia rat CA1 subfield before operating. Serial cryo-sections of brain from rats were examined under microscope after ABC immunohis-tochemistrystaining. Results: NR2B expression was obviously decreased (P0. 01) after ANR2B injection in CA1 subfield, but SNR2B. NS injection did not change NR2B expression. Conclusion: Expression of NR2B can be decreased in vivo by the antisense oligonucleotide technique.

Key concepts: Hippocampal formation, Forebrain, NMDA receptor, Oligonucleotide, Ischemia, In vivo, Messenger RNA, Hippocampus

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Effect of antisense oligonucleotide on expression of NR2B in hippocampal CAl following transient forebrain ischemia — Research Paper | ScholarLens