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Effects of curcumin on PI3K/AKT signaling pathway in HL60 Cells

Gao Qingping

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Abstract

Objective To investigate the effects of curcumin on the PI3K/AKT signaling pathway in HL60 cells and the apoptosis of HL60 cells. Methods The inhibitory effects of curcumin on the proliferation of HL60 cells were determined by MTT assay. After being treated by curcumin, the morphological and structural changes of the cells were observed by inverted microscope. The levels of CAV-1, AKT and p-AKT protein were measured by Western blot. Results MTT assay revealed that curcumin has growth-inhibiting effects on HL60 cells, and the effects were enhanced with the increase of concentration. Apoptotic morphology changes were observed by inverted microscope. The level of CAV-1 protein was increased, while the levels of AKT and p-AKT protein were reduced. Conclusion Curcumin can inhibit PI3K/AKT signaling pathway by upregulating the expression of CAV-1, resulting in inhibiting cell proliferation and promoting apoptosis in HL60 cells.

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What this paper is about

Objective To investigate the effects of curcumin on the PI3K/AKT signaling pathway in HL60 cells and the apoptosis of HL60 cells. Methods The inhibitory effects of curcumin on the proliferation of HL60 cells were determined by MTT assay. After being treated by curcumin, the morphological and structural changes of the cells were observed by inverted microscope. The levels of CAV-1, AKT and p-AKT protein were measured by Western blot. Results MTT assay revealed that curcumin has growth-inhibiting effects on HL60 cells, and the effects were enhanced with the increase of concentration. Apoptotic morphology changes were observed by inverted microscope. The level of CAV-1 protein was increased, while the levels of AKT and p-AKT protein were reduced. Conclusion Curcumin can inhibit PI3K/AKT signaling pathway by upregulating the expression of CAV-1, resulting in inhibiting cell proliferation and promoting apoptosis in HL60 cells.

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Available abstract

Objective To investigate the effects of curcumin on the PI3K/AKT signaling pathway in HL60 cells and the apoptosis of HL60 cells. Methods The inhibitory effects of curcumin on the proliferation of HL60 cells were determined by MTT assay. After being treated by curcumin, the morphological and structural changes of the cells were observed by inverted microscope. The levels of CAV-1, AKT and p-AKT protein were measured by Western blot. Results MTT assay revealed that curcumin has growth-inhibiting effects on HL60 cells, and the effects were enhanced with the increase of concentration. Apoptotic morphology changes were observed by inverted microscope. The level of CAV-1 protein was increased, while the levels of AKT and p-AKT protein were reduced. Conclusion Curcumin can inhibit PI3K/AKT signaling pathway by upregulating the expression of CAV-1, resulting in inhibiting cell proliferation and promoting apoptosis in HL60 cells.

Key concepts: Curcumin, Protein kinase B, PI3K/AKT/mTOR pathway, Apoptosis, Western blot, Cell biology, MTT assay, HL60

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